|
| Status |
Public on May 07, 2013 |
| Title |
RA_SF_individual1 |
| Sample type |
genomic |
| |
|
| Source name |
Rheumathoid Arthritis Synovial Fibroblast
|
| Organism |
Homo sapiens |
| Characteristics |
cell type: Synovial Fibroblast
diagnosis: Rheumatoid arthritis
|
| Treatment protocol |
no treatment
|
| Growth protocol |
Fibroblast cultures were maintained in Dulbecco's modified Eagle's medium supplemented with 10% fetal calf serum
|
| Extracted molecule |
genomic DNA |
| Extraction protocol |
Proteinase K extraction
|
| Label |
Cy5 and Cy3
|
| Label protocol |
Standard Illumina Protocol
|
| |
|
| Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Infinium Human Methylation450k Beadchip using standard Illumina protocol
|
| Scan protocol |
Arrays were imaged using BeadArray Reader (Illumina HiSeq2000) using standard recommended Illumina scanner setting
|
| Description |
Rheumathoid Arthritis Synovial Fibroblast
|
| Data processing |
Regular BeadStudio processing
|
| |
|
| Submission date |
May 06, 2013 |
| Last update date |
May 07, 2013 |
| Contact name |
Esteban Ballestar |
| Organization name |
Josep Carreras Research Institute (IJC)
|
| Lab |
Epigenetics and Immune Disease
|
| Street address |
Ctra de Can Ruti, Camà de les Escoles s/n
|
| City |
Badalona, Barcelona |
| State/province |
N/A = Not Applicable |
| ZIP/Postal code |
08916 |
| Country |
Spain |
| |
|
| Platform ID |
GPL13534 |
| Series (1) |
| GSE46650 |
Identification of novel markers in rheumatoid arthritis through integrated analysis of DNA methylation and microRNA expression |
|
