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Sample GSM1132972 Query DataSets for GSM1132972
Status Public on Dec 01, 2013
Title ES_2
Sample type RNA
Source name ES cells
Organism Mus musculus
Characteristics genotype: wildtype
cell type: embryonic stem cells
genetic background: C57BL/6
Treatment protocol GS and ES cells were treated with trypsin and collected.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Trizol (Invutrogen) and purified by RNAeasy column (QIAGEN).
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 0.2 ug RNA using the Low Input Quick Amp Labeling kit (Agilent) according to the manufacturer's instructions. Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
Hybridization protocol Cy3-labeled RNA probes were hybridized to a whole mouse genome DNA oligo microarray (4 × 44K, Agilent) for 16 hours at 65°C according to the manufacturer’s instructions.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner at a 5 um resolution.
Description Gene expression of ES cells
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent).
Submission date May 02, 2013
Last update date Dec 01, 2013
Contact name Jiyoung Lee
Organization name Tokyo Medical and Dental University
Street address 1-5-45 Yushima
City Bunkyo-ku
State/province Tokyo
ZIP/Postal code 113-8510
Country Japan
Platform ID GPL11202
Series (1)
GSE46589 Comparison of gene expression between Xist KO GS cells and wild type GS

Data table header descriptions
VALUE Default Agilent Feature Extraction normalized signal intensity

Data table
A_51_P100174 1213.667
A_51_P100298 102.5698
A_51_P100309 18.81156
A_51_P100327 29.34678
A_51_P100537 30.28964
A_51_P100573 321.4135
A_51_P100625 60.14283
A_51_P100776 52.64625
A_51_P100787 3921.905
A_51_P100828 3197.397
A_51_P100852 68.53388
A_51_P100997 112.1139
A_51_P101006 144.0758
A_51_P101075 3617.132
A_51_P101196 7031.214
A_51_P101228 268.2357
A_51_P101375 44.39218
A_51_P101460 63.85153
A_51_P101660 258.9736
A_51_P102122 10.9079

Total number of rows: 39429

Table truncated, full table size 865 Kbytes.

Supplementary file Size Download File type/resource
GSM1132972_US45102915_SLOT02_S01_GE1_1100_Jul11_1_4.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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