|Public on Dec 01, 2013
|Xist KO GS_1
|genotype: Xist KO
cell type: germ line stem cells
genetic background: DBA/2
|GS and ES cells were treated with trypsin and collected.
|Total RNA was isolated using Trizol (Invutrogen) and purified by RNAeasy column (QIAGEN).
|Cyanine-3 (Cy3) labeled cRNA was prepared from 0.2 ug RNA using the Low Input Quick Amp Labeling kit (Agilent) according to the manufacturer's instructions. Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
|Cy3-labeled RNA probes were hybridized to a whole mouse genome DNA oligo microarray (4 × 44K, Agilent) for 16 hours at 65°C according to the manufacturer’s instructions.
|Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner at a 5 um resolution.
|Gene expression of Xist KO GS cells
|The scanned images were analyzed with Feature Extraction Software (Agilent).
|May 02, 2013
|Last update date
|Dec 01, 2013
|Tokyo Medical and Dental University
|Comparison of gene expression between Xist KO GS cells and wild type GS