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Sample GSM1132967 Query DataSets for GSM1132967
Status Public on Dec 01, 2013
Title wt GS_3
Sample type RNA
Source name GS cells
Organism Mus musculus
Characteristics genotype: wildtype
cell type: germ line stem cells
genetic background: DBA/2
Treatment protocol GS and ES cells were treated with trypsin and collected.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Trizol (Invutrogen) and purified by RNAeasy column (QIAGEN).
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 0.2 ug RNA using the Low Input Quick Amp Labeling kit (Agilent) according to the manufacturer's instructions. Dye incorporation and cRNA yield were checked with the NanoDrop ND-1000 Spectrophotometer.
Hybridization protocol Cy3-labeled RNA probes were hybridized to a whole mouse genome DNA oligo microarray (4 × 44K, Agilent) for 16 hours at 65°C according to the manufacturer’s instructions.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner at a 5 um resolution.
Description Gene expression of wildtype GS cells
Data processing The scanned images were analyzed with Feature Extraction Software (Agilent).
Submission date May 02, 2013
Last update date Dec 01, 2013
Contact name Jiyoung Lee
Organization name Tokyo Medical and Dental University
Street address 1-5-45 Yushima
City Bunkyo-ku
State/province Tokyo
ZIP/Postal code 113-8510
Country Japan
Platform ID GPL11202
Series (1)
GSE46589 Comparison of gene expression between Xist KO GS cells and wild type GS

Data table header descriptions
VALUE Default Agilent Feature Extraction normalized signal intensity

Data table
A_51_P100174 5131.407
A_51_P100298 122.2539
A_51_P100309 6.476146
A_51_P100327 14.07644
A_51_P100537 14.40436
A_51_P100573 274.8924
A_51_P100625 9.673662
A_51_P100776 116.7602
A_51_P100787 5528.454
A_51_P100828 775.599
A_51_P100852 12.59317
A_51_P100997 78.28667
A_51_P101006 1029.88
A_51_P101075 27946.65
A_51_P101196 12777
A_51_P101228 231.4479
A_51_P101375 86.975
A_51_P101460 22.29499
A_51_P101660 193.7825
A_51_P102122 53.61273

Total number of rows: 39429

Table truncated, full table size 865 Kbytes.

Supplementary file Size Download File type/resource
GSM1132967_US45102915_SLOT01_S01_GE1_1100_Jul11_1_3.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table
Processed data provided as supplementary file

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