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Sample GSM1131294 Query DataSets for GSM1131294
Status Public on Nov 04, 2013
Title fasted, 24h, white adipose tissue, biological replicate 2
Sample type RNA
 
Source name epididymal white adipose tissue from C57BL/6 mice, fasted, 24h
Organism Mus musculus
Characteristics gender: male
strain: C57Bl/6
age: 10-12 weeks
tissue: epididymal white adipose tissue
Treatment protocol Food was withdrawn from the fasting group (n=5) for 24 hours.
Growth protocol mice were kept under standard conditions according to the institutional guidelines of the University of Pennsylvania. Mice were kept with normal chow diet in a 12h day/ 12h night cycle.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 150ng total RNA.
 
Hybridization protocol Genechip hybridization, wash, stain and scan were performed using the GeneTitan hyb-wash-stain kit (Affymetrix) and GeneTitan Instrument.
Scan protocol GeneTitan instrument was used for scanning.
Description gene expression of white adipose tissue of fasted (24h) male C57Bl/6 mice
Data processing Raw array data were automatically collected by GeneTitan. Data were normalized using default RMA method within Partek Genomics Suite software. A two-way ANOVA model with an interaction term between diet (fed or fasted) and tissue (liver, skeletal muscle and white adipose tissue) was set up. Pairwise comparisons were made between fed and fasted diet for each tissue. The resulting p-values of significance were corrected for multiple testing using Benjamini-Hochberg's false discovery rate (FDR) method. Genes within 5% FDR and changed at least by 1.3-fold in either direction were called differentially expressed.
 
Submission date Apr 30, 2013
Last update date Nov 04, 2013
Contact name Andreas Prokesch
E-mail(s) andreas.prokesch@medunigraz.at
Organization name Medical University Graz
Department Institute of Cell Biology, Histology, and Embryology
Street address Harrachgasse 21/7
City Graz
State/province Styria
ZIP/Postal code 8010
Country Austria
 
Platform ID GPL11533
Series (1)
GSE46495 Transcriptome signature of white adipose tissue, liver, and skeletal muscle in 24 hours fasted mice (C57Bl/6J)

Data table header descriptions
ID_REF
VALUE RMA normalized log2 intensities

Data table
ID_REF VALUE
10605815 3.58401
10390103 11.2281
10406823 6.05094
10424979 5.91092
10447951 10.761
10559790 9.20934
10442625 4.65604
10543017 11.8491
10469867 9.69747
10458046 6.63198
10505779 10.625
10443730 10.237
10544148 10.4923
10476383 9.0438
10503259 10.5303
10529858 10.1738
10495562 5.915
10472212 10.2622
10556456 10.3272
10355567 11.8388

Total number of rows: 28815

Table truncated, full table size 475 Kbytes.




Supplementary file Size Download File type/resource
GSM1131294_3502_19477_fastedF2_MoGene1_1ST.CEL.gz 4.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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