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Sample GSM1131281 Query DataSets for GSM1131281
Status Public on Nov 04, 2013
Title ad libitum chow, 24h, white adipose tissue, biological replicate 4
Sample type RNA
 
Source name epididymal white adipose tissue from C57BL/6 mice, ad libitum, 24h
Organism Mus musculus
Characteristics gender: male
strain: C57Bl/6
age: 10-12 weeks
tissue: epididymal white adipose tissue
Treatment protocol Food was withdrawn from the fasting group (n=5) for 24 hours.
Growth protocol mice were kept under standard conditions according to the institutional guidelines of the University of Pennsylvania. Mice were kept with normal chow diet in a 12h day/ 12h night cycle.
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 150ng total RNA.
 
Hybridization protocol Genechip hybridization, wash, stain and scan were performed using the GeneTitan hyb-wash-stain kit (Affymetrix) and GeneTitan Instrument.
Scan protocol GeneTitan instrument was used for scanning.
Description gene expression of white adipose tissue of ad libitum fed male C57Bl/6 mice
Data processing Raw array data were automatically collected by GeneTitan. Data were normalized using default RMA method within Partek Genomics Suite software. A two-way ANOVA model with an interaction term between diet (fed or fasted) and tissue (liver, skeletal muscle and white adipose tissue) was set up. Pairwise comparisons were made between fed and fasted diet for each tissue. The resulting p-values of significance were corrected for multiple testing using Benjamini-Hochberg's false discovery rate (FDR) method. Genes within 5% FDR and changed at least by 1.3-fold in either direction were called differentially expressed.
 
Submission date Apr 30, 2013
Last update date Nov 04, 2013
Contact name Andreas Prokesch
E-mail(s) andreas.prokesch@medunigraz.at
Organization name Medical University Graz
Department Institute of Cell Biology, Histology, and Embryology
Street address Harrachgasse 21/7
City Graz
State/province Styria
ZIP/Postal code 8010
Country Austria
 
Platform ID GPL11533
Series (1)
GSE46495 Transcriptome signature of white adipose tissue, liver, and skeletal muscle in 24 hours fasted mice (C57Bl/6J)

Data table header descriptions
ID_REF
VALUE RMA normalized log2 intensities

Data table
ID_REF VALUE
10605815 7.65653
10390103 9.90152
10406823 4.20076
10424979 8.08109
10447951 7.73281
10559790 5.90154
10442625 6.93004
10543017 9.1938
10469867 7.91143
10458046 8.78235
10505779 8.7812
10443730 7.89385
10544148 9.02394
10476383 7.45557
10503259 8.31128
10529858 9.31612
10495562 8.31527
10472212 9.07366
10556456 9.11524
10355567 10.8711

Total number of rows: 28815

Table truncated, full table size 475 Kbytes.




Supplementary file Size Download File type/resource
GSM1131281_3502_19464_fedF4_MoGene1_1ST.CEL.gz 4.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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