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Sample GSM1130167 Query DataSets for GSM1130167
Status Public on Jul 08, 2013
Title non-TPC Cells_rep4
Sample type RNA
 
Source name freshly FACS-sorted mouse non-TPC cells
Organism Mus musculus
Characteristics tissue: lung adenocarcinoma
cell type: non-TPC
genotype: KrasG12D, Trp53fl/fl
Treatment protocol Primary mouse lung adenocarcinoma cells were FACS-sorted into TPC(CD24+ITGB4+Notch(hi)) and non-TPC(remainder of tumor cells) populations.Cells pellets were kept in RNAlater and stored at -80C
Extracted molecule total RNA
Extraction protocol RNA were extracted by using RNAeasy microKit (Qiagen) following the manufacturer's recommendations, Ten to fifty nanograms of total RNA were amplified by two rounds of cDNA synthesis using Message Amp II aRNA Amplification kit (Applied Biosystems, Foster City, CA).
Label Cy5
Label protocol IVT reaction was performed using the Agilent Quick Amp Labeling kit (Agilent Technologies, Palo Alto, CA) to label cDNAs with Cy-5.
 
Hybridization protocol The Cy-5 labeled test sample was pooled with a Cy-3 labeled Universal Mouse Reference RNA (Agilent Technologies, Palo Alto, CA) and hybridized onto Agilent Whole Mouse Genome 4x44K arrays as described in manufacturer’s protocol
Scan protocol Arrays were washed, dried and scanned using an Agilent DNA microarray scanner.
Description Gene expression in non-TPC lung tumor cells with KrasG12D, Trp53fl/fl genotype
Data processing Agilent Feature Extraction software 9.5 was used to analyze acquired array images and obtain individual log2 ratios of background-subtracted signal intensities.
 
Submission date Apr 26, 2013
Last update date Jul 08, 2013
Contact name Yanyan Zheng
E-mail(s) yanyanzh@stanford.edu
Organization name Stanford University
Street address 265 Campus Drive
City Stanford
ZIP/Postal code 94305
Country USA
 
Platform ID GPL7202
Series (1)
GSE46439 Expression profiling of lung adenocarcinoma TPC tumor cells in Kras-driven NSCLC mouse model

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner -1.237318
DarkCorner -0.7100878
A_52_P616356 -0.7475884
A_52_P580582 -0.48822224
A_52_P403405 1.8443302
A_52_P819156 0.5588889
A_51_P331831 -2.5174267
A_51_P430630 0.2829976
A_52_P502357 -0.73435766
A_52_P299964 -1.4350677
A_51_P356389 -1.0315641
A_52_P684402 -0.64006996
A_51_P414208 1.6205174
A_51_P280918 -0.43415254
A_52_P613688 0.800806
A_52_P258194 -0.99300486
A_52_P229271 -2.476859
A_52_P214630 2.4488668
A_52_P579519 1.2592516
A_52_P979997 -0.41557235

Total number of rows: 41267

Table truncated, full table size 953 Kbytes.




Supplementary file Size Download File type/resource
GSM1130167_Agilent_251486838236_S001_GE2_1100_Jul11_1_4.txt.gz 4.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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