|
| Status |
Public on Jul 08, 2013 |
| Title |
non-TPC Cells_rep1 |
| Sample type |
RNA |
| |
|
| Source name |
freshly FACS-sorted mouse non-TPC cells
|
| Organism |
Mus musculus |
| Characteristics |
tissue: lung adenocarcinoma
cell type: non-TPC
genotype: KrasG12D, Trp53fl/fl
|
| Treatment protocol |
Primary mouse lung adenocarcinoma cells were FACS-sorted into TPC(CD24+ITGB4+Notch(hi)) and non-TPC(remainder of tumor cells) populations.Cells pellets were kept in RNAlater and stored at -80C
|
| Extracted molecule |
total RNA |
| Extraction protocol |
RNA were extracted by using RNAeasy microKit (Qiagen) following the manufacturer's recommendations, Ten to fifty nanograms of total RNA were amplified by two rounds of cDNA synthesis using Message Amp II aRNA Amplification kit (Applied Biosystems, Foster City, CA).
|
| Label |
Cy5
|
| Label protocol |
IVT reaction was performed using the Agilent Quick Amp Labeling kit (Agilent Technologies, Palo Alto, CA) to label cDNAs with Cy-5.
|
| |
|
| Hybridization protocol |
The Cy-5 labeled test sample was pooled with a Cy-3 labeled Universal Mouse Reference RNA (Agilent Technologies, Palo Alto, CA) and hybridized onto Agilent Whole Mouse Genome 4x44K arrays as described in manufacturer’s protocol
|
| Scan protocol |
Arrays were washed, dried and scanned using an Agilent DNA microarray scanner.
|
| Description |
Gene expression in non-TPC lung tumor cells with KrasG12D, Trp53fl/fl genotype
|
| Data processing |
Agilent Feature Extraction software 9.5 was used to analyze acquired array images and obtain individual log2 ratios of background-subtracted signal intensities.
|
| |
|
| Submission date |
Apr 26, 2013 |
| Last update date |
Jul 08, 2013 |
| Contact name |
Yanyan Zheng |
| E-mail(s) |
yanyanzh@stanford.edu
|
| Organization name |
Stanford University
|
| Street address |
265 Campus Drive
|
| City |
Stanford |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platform ID |
GPL7202 |
| Series (1) |
| GSE46439 |
Expression profiling of lung adenocarcinoma TPC tumor cells in Kras-driven NSCLC mouse model |
|
