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Sample GSM1130160 Query DataSets for GSM1130160
Status Public on Jul 08, 2013
Title TPC Cells_rep3
Sample type RNA
 
Source name freshly FACS-sorted mouse TPC cells
Organism Mus musculus
Characteristics tissue: lung adenocarcinoma
cell type: TPC
genotype: KrasG12D, Trp53fl/fl
Treatment protocol Primary mouse lung adenocarcinoma cells were FACS-sorted into TPC(CD24+ITGB4+Notch(hi)) and non-TPC(remainder of tumor cells) populations.Cells pellets were kept in RNAlater and stored at -80C
Extracted molecule total RNA
Extraction protocol RNA were extracted by using RNAeasy microKit (Qiagen) following the manufacturer's recommendations, Ten to fifty nanograms of total RNA were amplified by two rounds of cDNA synthesis using Message Amp II aRNA Amplification kit (Applied Biosystems, Foster City, CA).
Label Cy5
Label protocol IVT reaction was performed using the Agilent Quick Amp Labeling kit (Agilent Technologies, Palo Alto, CA) to label cDNAs with Cy-5.
 
Hybridization protocol The Cy-5 labeled test sample was pooled with a Cy-3 labeled Universal Mouse Reference RNA (Agilent Technologies, Palo Alto, CA) and hybridized onto Agilent Whole Mouse Genome 4x44K arrays as described in manufacturer’s protocol
Scan protocol Arrays were washed, dried and scanned using an Agilent DNA microarray scanner.
Description Gene expression in lung TPC cells with KrasG12D, Trp53fl/fl genotype
Data processing Agilent Feature Extraction software 9.5 was used to analyze acquired array images and obtain individual log2 ratios of background-subtracted signal intensities.
 
Submission date Apr 26, 2013
Last update date Jul 08, 2013
Contact name Yanyan Zheng
E-mail(s) yanyanzh@stanford.edu
Organization name Stanford University
Street address 265 Campus Drive
City Stanford
ZIP/Postal code 94305
Country USA
 
Platform ID GPL7202
Series (1)
GSE46439 Expression profiling of lung adenocarcinoma TPC tumor cells in Kras-driven NSCLC mouse model

Data table header descriptions
ID_REF
VALUE Normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner -1.2127608
DarkCorner -0.14409995
A_52_P616356 -0.15772218
A_52_P580582 0.27608976
A_52_P403405 2.7234576
A_52_P819156 1.246273
A_51_P331831 -2.074006
A_51_P430630 1.8085864
A_52_P502357 -0.15135834
A_52_P299964 0.3181526
A_51_P356389 -0.28132436
A_52_P684402 -0.61444855
A_51_P414208 1.1212978
A_51_P280918 -0.8284752
A_52_P613688 0.32103738
A_52_P258194 -0.7514716
A_52_P229271 -3.0375926
A_52_P214630 2.1366596
A_52_P579519 1.2645339
A_52_P979997 -0.14410186

Total number of rows: 41267

Table truncated, full table size 957 Kbytes.




Supplementary file Size Download File type/resource
GSM1130160_Agilent_251486838236_S001_GE2_1100_Jul11_1_1.txt.gz 4.4 Mb (ftp)(http) TXT
Processed data included within Sample table

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