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Sample GSM1129832 Query DataSets for GSM1129832
Status Public on Dec 31, 2013
Title A673/TR/LOX-PP_replicate_3
Sample type RNA
 
Source name A673/TR/LOX-PP, doxycycline stimulated, 72 hours
Organism Homo sapiens
Characteristics cell line: A673 cell line (Ewing sarcoma)
genotype/variation: LOX-PP induced expression
Treatment protocol Cells were stimulated with doxycycline (1 uM) for 72 hours to induce the expression of LOX-PP
Growth protocol Cells were grown in DMEM supplemented with 10% serum and antibiotics.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Qiagen RNeasy mini kit. Quality and integrity of the RNA was determined with a Bioanalyzer.
Label Cy3
Label protocol According to Agilent instructions (One-Color Microarray-based gene expression analysis v6.5)
 
Hybridization protocol According to Agilent instructions (One-Color Microarray-based gene expression analysis v6.5)
Scan protocol According to Agilent instructions (One-Color Microarray-based gene expression analysis v6.5)
Description LOX-PP 3
Data processing The scanned images were analyzed with Feature Extraction Software 10.7 (Agilent) using default parameters.
 
Submission date Apr 26, 2013
Last update date Dec 31, 2013
Contact name Javier Alonso
E-mail(s) fjalonso@isciii.es
Phone 34-91-8223217
Organization name INSTITUTO DE SALUD CARLOS III - IIER
Department AREA DE GENÉTICA HUMANA
Lab UNIDAD DE TUMORES SOLIDOS INFANTILES
Street address CTRA. MAJADAHONDA-POZUELO KM 2
City MAJADAHONDA
State/province MADRID
ZIP/Postal code 28220
Country Spain
 
Platform ID GPL17077
Series (1)
GSE46407 Effect of LOX-PP on the gene expression profile of the A673 cell line (Ewing sarcoma)

Data table header descriptions
ID_REF
VALUE log2 normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 0.7280507
DarkCorner 0.59146833
A_23_P117082 0.69866896
A_33_P3246448 -0.18896008
A_33_P3318220 -0.07343483
A_33_P3236322 0.20816803
A_33_P3319925 0.3725791
A_21_P0000509 0.8258343
A_21_P0000744 0.29134512
A_24_P215804 0.6708622
A_23_P110167 -0.69526434
A_33_P3211513 -0.66530514
A_23_P103349 -0.30633163
A_32_P61480 -0.20740128
A_33_P3788124 0.35497522
A_33_P3414202 -0.6447568
A_33_P3316686 -0.49047613
A_33_P3300975 -0.4733305
A_33_P3263061 0.1522665
A_33_P3261373 -0.2121296

Total number of rows: 50739

Table truncated, full table size 1219 Kbytes.




Supplementary file Size Download File type/resource
GSM1129832_US82003505_253949410585_S02_GE1_107_Sep09_2_4.txt.gz 3.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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