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Sample GSM1129830 Query DataSets for GSM1129830
Status Public on Dec 31, 2013
Title A673/TR/LOX-PP_replicate_1
Sample type RNA
 
Source name A673/TR/LOX-PP, doxycycline stimulated, 72 hours
Organism Homo sapiens
Characteristics cell line: A673 cell line (Ewing sarcoma)
genotype/variation: LOX-PP induced expression
Treatment protocol Cells were stimulated with doxycycline (1 uM) for 72 hours to induce the expression of LOX-PP
Growth protocol Cells were grown in DMEM supplemented with 10% serum and antibiotics.
Extracted molecule total RNA
Extraction protocol Total RNA was isolated using Qiagen RNeasy mini kit. Quality and integrity of the RNA was determined with a Bioanalyzer.
Label Cy3
Label protocol According to Agilent instructions (One-Color Microarray-based gene expression analysis v6.5)
 
Hybridization protocol According to Agilent instructions (One-Color Microarray-based gene expression analysis v6.5)
Scan protocol According to Agilent instructions (One-Color Microarray-based gene expression analysis v6.5)
Description LOX-PP 1
Data processing The scanned images were analyzed with Feature Extraction Software 10.7 (Agilent) using default parameters.
 
Submission date Apr 26, 2013
Last update date Dec 31, 2013
Contact name Javier Alonso
E-mail(s) fjalonso@isciii.es
Phone 34-91-8223217
Organization name INSTITUTO DE SALUD CARLOS III - IIER
Department AREA DE GENÉTICA HUMANA
Lab UNIDAD DE TUMORES SOLIDOS INFANTILES
Street address CTRA. MAJADAHONDA-POZUELO KM 2
City MAJADAHONDA
State/province MADRID
ZIP/Postal code 28220
Country Spain
 
Platform ID GPL17077
Series (1)
GSE46407 Effect of LOX-PP on the gene expression profile of the A673 cell line (Ewing sarcoma)

Data table header descriptions
ID_REF
VALUE log2 normalized signal intensity

Data table
ID_REF VALUE
GE_BrightCorner 1.4502225
DarkCorner -1.1252661
A_23_P117082 -0.8034005
A_33_P3246448 0.640996
A_33_P3318220 -1.7954569
A_33_P3236322 -1.2324829
A_33_P3319925 -2.080358
A_21_P0000509 1.1393948
A_21_P0000744 -0.56091356
A_24_P215804 0.9002857
A_23_P110167 -1.2323194
A_33_P3211513 0.46141148
A_23_P103349 0.95774746
A_32_P61480 -1.9485264
A_33_P3788124 -2.1372666
A_33_P3414202 -0.00128603
A_33_P3316686 0.58424234
A_33_P3300975 -0.54132843
A_33_P3263061 -0.07410717
A_33_P3261373 -1.9142809

Total number of rows: 50739

Table truncated, full table size 1221 Kbytes.




Supplementary file Size Download File type/resource
GSM1129830_US82003505_253949410307_S01_GE1_107_Sep09_1_1.txt.gz 3.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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