|
Status |
Public on Dec 31, 2013 |
Title |
A673/TR/LOX-PP_replicate_1 |
Sample type |
RNA |
|
|
Source name |
A673/TR/LOX-PP, doxycycline stimulated, 72 hours
|
Organism |
Homo sapiens |
Characteristics |
cell line: A673 cell line (Ewing sarcoma) genotype/variation: LOX-PP induced expression
|
Treatment protocol |
Cells were stimulated with doxycycline (1 uM) for 72 hours to induce the expression of LOX-PP
|
Growth protocol |
Cells were grown in DMEM supplemented with 10% serum and antibiotics.
|
Extracted molecule |
total RNA |
Extraction protocol |
Total RNA was isolated using Qiagen RNeasy mini kit. Quality and integrity of the RNA was determined with a Bioanalyzer.
|
Label |
Cy3
|
Label protocol |
According to Agilent instructions (One-Color Microarray-based gene expression analysis v6.5)
|
|
|
Hybridization protocol |
According to Agilent instructions (One-Color Microarray-based gene expression analysis v6.5)
|
Scan protocol |
According to Agilent instructions (One-Color Microarray-based gene expression analysis v6.5)
|
Description |
LOX-PP 1
|
Data processing |
The scanned images were analyzed with Feature Extraction Software 10.7 (Agilent) using default parameters.
|
|
|
Submission date |
Apr 26, 2013 |
Last update date |
Dec 31, 2013 |
Contact name |
Javier Alonso |
E-mail(s) |
fjalonso@isciii.es
|
Phone |
34-91-8223217
|
Organization name |
INSTITUTO DE SALUD CARLOS III - IIER
|
Department |
AREA DE GENÉTICA HUMANA
|
Lab |
UNIDAD DE TUMORES SOLIDOS INFANTILES
|
Street address |
CTRA. MAJADAHONDA-POZUELO KM 2
|
City |
MAJADAHONDA |
State/province |
MADRID |
ZIP/Postal code |
28220 |
Country |
Spain |
|
|
Platform ID |
GPL17077 |
Series (1) |
GSE46407 |
Effect of LOX-PP on the gene expression profile of the A673 cell line (Ewing sarcoma) |
|