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Sample GSM1124572 Query DataSets for GSM1124572
Status Public on Apr 20, 2013
Title M_133A_41_HC_1_C
Sample type RNA
 
Source name Post-mortem human brain tissue: Hippocampus
Organism Homo sapiens
Characteristics genechip assay site: M
original genechip type: U133A
subject id: 41
brain region: hippocampus
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 4.691471651
10001_at 5.260366694
10002_at 5.061441939
10003_at 4.385789899
100048912_at 6.293943035
10004_at 5.196166826
10005_at 7.270156812
10006_at 7.327873814
10007_at 7.197631805
10009_at 4.176496939
1000_at 7.892182212
10010_at 5.10420298
100127886_at 4.082926932
100127972_at 4.822108268
100128008_at 7.983356998
100128062_at 7.783081914
100128414_at 4.066071942
100128919_at 6.244051457
100129015_at 4.775416551
100129128_at 4.483574808

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124572_M_133A_41_HC_1_C.cel.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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