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Sample GSM1124561 Query DataSets for GSM1124561
Status Public on Apr 20, 2013
Title D_133A_25_HC_1_C_p
Sample type RNA
 
Source name Post-mortem human brain tissue: Hippocampus
Organism Homo sapiens
Characteristics genechip assay site: D
original genechip type: U133P
subject id: 25
brain region: hippocampus
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 4.87351691
10001_at 5.053218123
10002_at 5.005957902
10003_at 4.692742652
100048912_at 6.030934113
10004_at 5.299197622
10005_at 6.875057971
10006_at 7.309407463
10007_at 6.986520713
10009_at 4.087096782
1000_at 8.020732359
10010_at 5.423248943
100127886_at 4.061080229
100127972_at 4.798394966
100128008_at 6.800168135
100128062_at 8.373607279
100128414_at 4.156961123
100128919_at 6.414001005
100129015_at 4.593140095
100129128_at 4.390617891

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124561_D_133A_25_HC_1_C_p.cel.gz 3.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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