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Sample GSM1124545 Query DataSets for GSM1124545
Status Public on Apr 20, 2013
Title D_133A_102_HC_1_C_p
Sample type RNA
 
Source name Post-mortem human brain tissue: Hippocampus
Organism Homo sapiens
Characteristics genechip assay site: D
original genechip type: U133P
subject id: 102
brain region: hippocampus
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 4.840249337
10001_at 5.275479066
10002_at 4.921996745
10003_at 4.551211056
100048912_at 5.754768649
10004_at 5.150089552
10005_at 7.129744325
10006_at 7.753449852
10007_at 7.036207672
10009_at 4.219445349
1000_at 7.896213763
10010_at 5.341756289
100127886_at 4.162880998
100127972_at 4.813906948
100128008_at 6.540812632
100128062_at 8.410305051
100128414_at 4.248289684
100128919_at 6.828339551
100129015_at 4.461866024
100129128_at 4.495067167

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124545_D_133A_102_HC_1_C_p.cel.gz 3.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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