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Sample GSM1124523 Query DataSets for GSM1124523
Status Public on Apr 20, 2013
Title D_133A_45_HC_1_C
Sample type RNA
 
Source name Post-mortem human brain tissue: Hippocampus
Organism Homo sapiens
Characteristics genechip assay site: D
original genechip type: U133A
subject id: 45
brain region: hippocampus
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 4.902402159
10001_at 5.143397056
10002_at 5.042694193
10003_at 4.397983191
100048912_at 6.427011359
10004_at 5.474444593
10005_at 7.581681069
10006_at 6.059436487
10007_at 6.582798439
10009_at 4.146478768
1000_at 7.555693407
10010_at 5.116709866
100127886_at 4.422079031
100127972_at 5.203426273
100128008_at 6.962960584
100128062_at 7.738815698
100128414_at 4.219998469
100128919_at 6.715654965
100129015_at 4.926659548
100129128_at 4.354753508

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124523_D_133A_45_HC_1_C.cel.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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