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Sample GSM1124517 Query DataSets for GSM1124517
Status Public on Apr 20, 2013
Title M_133A_125_DLPFC_1_C
Sample type RNA
 
Source name Post-mortem human brain tissue: Dorsolateral Prefrontal Cortex
Organism Homo sapiens
Characteristics genechip assay site: M
original genechip type: U133A
subject id: 125
brain region: dorsolateral prefrontal cortex
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.128831027
10001_at 5.589613067
10002_at 5.086053302
10003_at 4.434848477
100048912_at 6.755078968
10004_at 5.087522122
10005_at 7.15792255
10006_at 7.03164461
10007_at 7.375747889
10009_at 4.308207426
1000_at 7.910757867
10010_at 5.114284357
100127886_at 4.238108583
100127972_at 5.193538229
100128008_at 6.917231393
100128062_at 8.731136342
100128414_at 4.205010565
100128919_at 6.416734878
100129015_at 4.759822251
100129128_at 4.733704423

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124517_M_133A_125_DLPFC_1_C.cel.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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