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Sample GSM1124514 Query DataSets for GSM1124514
Status Public on Apr 20, 2013
Title M_133A_50_DLPFC_1_C
Sample type RNA
 
Source name Post-mortem human brain tissue: Dorsolateral Prefrontal Cortex
Organism Homo sapiens
Characteristics genechip assay site: M
original genechip type: U133A
subject id: 50
brain region: dorsolateral prefrontal cortex
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.24649545
10001_at 5.574290383
10002_at 5.231046701
10003_at 4.364958001
100048912_at 6.674701758
10004_at 5.357872371
10005_at 6.837963162
10006_at 6.964572199
10007_at 7.17231731
10009_at 4.289149088
1000_at 7.762442438
10010_at 5.032531325
100127886_at 4.195279367
100127972_at 5.14578171
100128008_at 7.057029247
100128062_at 8.438847409
100128414_at 4.253383288
100128919_at 6.740768862
100129015_at 4.814391543
100129128_at 4.712067417

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124514_M_133A_50_DLPFC_1_C.cel.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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