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Sample GSM1124502 Query DataSets for GSM1124502
Status Public on Apr 20, 2013
Title M_133A_8_DLPFC_1_C_p
Sample type RNA
 
Source name Post-mortem human brain tissue: Dorsolateral Prefrontal Cortex
Organism Homo sapiens
Characteristics genechip assay site: M
original genechip type: U133P
subject id: 8
brain region: dorsolateral prefrontal cortex
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 4.948279425
10001_at 6.163520815
10002_at 4.811334662
10003_at 4.674099034
100048912_at 6.033059991
10004_at 4.899393709
10005_at 7.438120276
10006_at 8.22504968
10007_at 8.070471948
10009_at 4.241678112
1000_at 7.569410204
10010_at 5.394692859
100127886_at 4.05503151
100127972_at 4.678513872
100128008_at 5.609638448
100128062_at 9.085833901
100128414_at 4.261335529
100128919_at 6.390035843
100129015_at 4.447265661
100129128_at 4.39812164

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124502_M_133A_8_DLPFC_1_C_p.cel.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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