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Sample GSM1124488 Query DataSets for GSM1124488
Status Public on Apr 20, 2013
Title M_133A_102_DLPFC_1_C
Sample type RNA
 
Source name Post-mortem human brain tissue: Dorsolateral Prefrontal Cortex
Organism Homo sapiens
Characteristics genechip assay site: M
original genechip type: U133A
subject id: 102
brain region: dorsolateral prefrontal cortex
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.037363144
10001_at 5.388956862
10002_at 5.251962594
10003_at 4.481723139
100048912_at 7.004660873
10004_at 5.23583405
10005_at 6.926438745
10006_at 6.94961396
10007_at 7.599841258
10009_at 4.301410007
1000_at 7.715243605
10010_at 4.941121074
100127886_at 4.079009738
100127972_at 4.998272541
100128008_at 7.533120231
100128062_at 7.705204676
100128414_at 4.287696989
100128919_at 6.023947147
100129015_at 4.943242071
100129128_at 4.802849203

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124488_M_133A_102_DLPFC_1_C.cel.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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