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Sample GSM1124487 Query DataSets for GSM1124487
Status Public on Apr 20, 2013
Title M_133A_30_DLPFC_1_C
Sample type RNA
 
Source name Post-mortem human brain tissue: Dorsolateral Prefrontal Cortex
Organism Homo sapiens
Characteristics genechip assay site: M
original genechip type: U133A
subject id: 30
brain region: dorsolateral prefrontal cortex
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.277571574
10001_at 5.448992
10002_at 5.220497022
10003_at 4.464196552
100048912_at 6.676723538
10004_at 5.132094238
10005_at 7.001074681
10006_at 6.461962778
10007_at 7.060657439
10009_at 4.310803498
1000_at 7.618137439
10010_at 5.019320851
100127886_at 4.237131027
100127972_at 5.296839787
100128008_at 7.589322135
100128062_at 7.9156534
100128414_at 4.291688574
100128919_at 6.14103329
100129015_at 4.765931709
100129128_at 4.810542402

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124487_M_133A_30_DLPFC_1_C.cel.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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