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Sample GSM1124486 Query DataSets for GSM1124486
Status Public on Apr 20, 2013
Title M_133A_32_DLPFC_1_C_r
Sample type RNA
 
Source name Post-mortem human brain tissue: Dorsolateral Prefrontal Cortex
Organism Homo sapiens
Characteristics genechip assay site: M
original genechip type: U133A
subject id: 32
brain region: dorsolateral prefrontal cortex
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.313814746
10001_at 5.554108664
10002_at 5.137895196
10003_at 4.451754836
100048912_at 6.626869655
10004_at 5.044473293
10005_at 6.998157748
10006_at 7.05562539
10007_at 7.80154101
10009_at 4.274822723
1000_at 7.686560041
10010_at 5.017331059
100127886_at 4.072486759
100127972_at 4.941746669
100128008_at 7.869865257
100128062_at 8.304600718
100128414_at 4.230394671
100128919_at 5.946415034
100129015_at 4.862461981
100129128_at 4.674654476

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124486_M_133A_32_DLPFC_1_C_r.cel.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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