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Sample GSM1124485 Query DataSets for GSM1124485
Status Public on Apr 20, 2013
Title M_133A_32_DLPFC_1_C
Sample type RNA
 
Source name Post-mortem human brain tissue: Dorsolateral Prefrontal Cortex
Organism Homo sapiens
Characteristics genechip assay site: M
original genechip type: U133A
subject id: 32
brain region: dorsolateral prefrontal cortex
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.165502006
10001_at 5.61290099
10002_at 5.079468155
10003_at 4.310287172
100048912_at 6.857701882
10004_at 5.097687137
10005_at 6.701398691
10006_at 7.072261852
10007_at 7.696384628
10009_at 4.337523082
1000_at 7.648595042
10010_at 5.02668755
100127886_at 4.135908066
100127972_at 5.029720256
100128008_at 7.578291025
100128062_at 8.035839773
100128414_at 4.235421334
100128919_at 6.073485003
100129015_at 4.818256888
100129128_at 4.583659236

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124485_M_133A_32_DLPFC_1_C.cel.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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