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Sample GSM1124457 Query DataSets for GSM1124457
Status Public on Apr 20, 2013
Title M_133A_51_DLPFC_1_C
Sample type RNA
 
Source name Post-mortem human brain tissue: Dorsolateral Prefrontal Cortex
Organism Homo sapiens
Characteristics genechip assay site: M
original genechip type: U133A
subject id: 51
brain region: dorsolateral prefrontal cortex
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.110793163
10001_at 5.690631349
10002_at 5.053955572
10003_at 4.560697948
100048912_at 6.667484893
10004_at 5.318949469
10005_at 7.396755391
10006_at 7.36890656
10007_at 7.300367926
10009_at 4.314916561
1000_at 7.9241852
10010_at 5.044720105
100127886_at 4.197438997
100127972_at 5.162873836
100128008_at 6.395988576
100128062_at 8.553902553
100128414_at 4.275139134
100128919_at 6.786315611
100129015_at 4.814933653
100129128_at 4.628814946

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124457_M_133A_51_DLPFC_1_C.cel.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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