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Sample GSM1124440 Query DataSets for GSM1124440
Status Public on Apr 20, 2013
Title D_133A_22_DLPFC_1_C_p
Sample type RNA
 
Source name Post-mortem human brain tissue: Dorsolateral Prefrontal Cortex
Organism Homo sapiens
Characteristics genechip assay site: D
original genechip type: U133P
subject id: 22
brain region: dorsolateral prefrontal cortex
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.187321388
10001_at 5.614423456
10002_at 5.016291291
10003_at 4.744418035
100048912_at 5.923925689
10004_at 4.970658085
10005_at 6.947743392
10006_at 7.785472032
10007_at 7.305765503
10009_at 4.259038488
1000_at 7.618448969
10010_at 5.295772758
100127886_at 4.013908066
100127972_at 4.735113929
100128008_at 6.462093385
100128062_at 8.618067975
100128414_at 4.204661244
100128919_at 6.864671977
100129015_at 4.52279162
100129128_at 4.541854594

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124440_D_133A_22_DLPFC_1_C_p.cel.gz 3.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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