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Sample GSM1124388 Query DataSets for GSM1124388
Status Public on Apr 20, 2013
Title D_133A_121_DLPFC_1_C
Sample type RNA
 
Source name Post-mortem human brain tissue: Dorsolateral Prefrontal Cortex
Organism Homo sapiens
Characteristics genechip assay site: D
original genechip type: U133A
subject id: 121
brain region: dorsolateral prefrontal cortex
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.024640861
10001_at 5.264978895
10002_at 5.201303348
10003_at 4.389463452
100048912_at 5.909110454
10004_at 5.605461583
10005_at 7.49947115
10006_at 7.092550216
10007_at 6.953739158
10009_at 4.228233757
1000_at 7.668331502
10010_at 5.06270078
100127886_at 4.391638213
100127972_at 5.021459584
100128008_at 6.73014975
100128062_at 8.313688489
100128414_at 4.260245737
100128919_at 6.571447782
100129015_at 4.992912603
100129128_at 4.493386586

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124388_D_133A_121_DLPFC_1_C.cel.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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