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Sample GSM1124336 Query DataSets for GSM1124336
Status Public on Apr 20, 2013
Title M_133A_22_CB_1_C_p
Sample type RNA
 
Source name Post-mortem human brain tissue: Cerebellum
Organism Homo sapiens
Characteristics genechip assay site: M
original genechip type: U133P
subject id: 22
brain region: cerebellum
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.725304697
10001_at 6.53028968
10002_at 5.995626793
10003_at 4.826245883
100048912_at 6.487288176
10004_at 5.917844051
10005_at 6.554682861
10006_at 8.08185047
10007_at 7.222110054
10009_at 4.665968665
1000_at 7.993521107
10010_at 6.114163474
100127886_at 4.576515386
100127972_at 5.208819894
100128008_at 7.389773511
100128062_at 10.29079772
100128414_at 4.681331341
100128919_at 6.67544594
100129015_at 5.289906089
100129128_at 4.979958059

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124336_M_133A_22_CB_1_C_p.cel.gz 3.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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