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Sample GSM1124315 Query DataSets for GSM1124315
Status Public on Apr 20, 2013
Title I_133A_28_CB_1_C_p
Sample type RNA
 
Source name Post-mortem human brain tissue: Cerebellum
Organism Homo sapiens
Characteristics genechip assay site: I
original genechip type: U133P
subject id: 28
brain region: cerebellum
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.30306482
10001_at 7.502205189
10002_at 5.446592828
10003_at 4.833559955
100048912_at 6.113319072
10004_at 5.534877232
10005_at 7.146942096
10006_at 8.904498254
10007_at 7.539640478
10009_at 4.656539475
1000_at 7.751848905
10010_at 6.272193209
100127886_at 4.4838112
100127972_at 5.158036374
100128008_at 6.417963314
100128062_at 10.59852763
100128414_at 4.606496164
100128919_at 6.876719973
100129015_at 5.07471136
100129128_at 5.060430283

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124315_I_133A_28_CB_1_C_p.cel.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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