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Sample GSM1124299 Query DataSets for GSM1124299
Status Public on Apr 20, 2013
Title I_133A_36_CB_0_C
Sample type RNA
 
Source name Post-mortem human brain tissue: Cerebellum
Organism Homo sapiens
Characteristics genechip assay site: I
original genechip type: U133A
subject id: 36
brain region: cerebellum
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.516477753
10001_at 7.039703759
10002_at 5.647974922
10003_at 4.737029742
100048912_at 6.85165961
10004_at 5.725328884
10005_at 7.449149154
10006_at 8.023234762
10007_at 7.402117856
10009_at 4.645094676
1000_at 7.863149791
10010_at 5.973734032
100127886_at 4.613958998
100127972_at 5.425021941
100128008_at 6.612561259
100128062_at 10.49743856
100128414_at 4.574769603
100128919_at 7.246781131
100129015_at 5.149276105
100129128_at 5.297600061

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124299_I_133A_36_CB_0_C.cel.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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