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Sample GSM1124291 Query DataSets for GSM1124291
Status Public on Apr 20, 2013
Title I_133A_118_CB_0_C
Sample type RNA
 
Source name Post-mortem human brain tissue: Cerebellum
Organism Homo sapiens
Characteristics genechip assay site: I
original genechip type: U133A
subject id: 118
brain region: cerebellum
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.476173413
10001_at 7.005201887
10002_at 6.041431491
10003_at 4.714635579
100048912_at 6.886691103
10004_at 5.805922171
10005_at 7.306087011
10006_at 8.288752996
10007_at 7.285822635
10009_at 4.56107143
1000_at 7.741116693
10010_at 5.802878462
100127886_at 4.710143234
100127972_at 5.553618871
100128008_at 6.889610596
100128062_at 10.23048944
100128414_at 4.629044813
100128919_at 6.667770766
100129015_at 5.404990075
100129128_at 5.265350018

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124291_I_133A_118_CB_0_C.cel.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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