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Sample GSM1124285 Query DataSets for GSM1124285
Status Public on Apr 20, 2013
Title I_133A_45_CB_1_C
Sample type RNA
 
Source name Post-mortem human brain tissue: Cerebellum
Organism Homo sapiens
Characteristics genechip assay site: I
original genechip type: U133A
subject id: 45
brain region: cerebellum
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.692386859
10001_at 6.927044183
10002_at 5.94669266
10003_at 4.832184664
100048912_at 6.97602542
10004_at 5.729552816
10005_at 7.360287535
10006_at 7.784327948
10007_at 7.247313145
10009_at 4.609225568
1000_at 7.806285759
10010_at 5.914222761
100127886_at 4.675160997
100127972_at 5.402941937
100128008_at 7.292248827
100128062_at 10.65571022
100128414_at 4.611018003
100128919_at 6.922104071
100129015_at 5.521749656
100129128_at 5.419675533

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124285_I_133A_45_CB_1_C.cel.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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