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Sample GSM1124278 Query DataSets for GSM1124278
Status Public on Apr 20, 2013
Title D_133A_102_CB_1_C_p
Sample type RNA
 
Source name Post-mortem human brain tissue: Cerebellum
Organism Homo sapiens
Characteristics genechip assay site: D
original genechip type: U133P
subject id: 102
brain region: cerebellum
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.603835792
10001_at 7.159671994
10002_at 5.72964576
10003_at 4.721313245
100048912_at 6.743851683
10004_at 5.912286196
10005_at 7.018853576
10006_at 8.763992746
10007_at 7.749160608
10009_at 4.730119365
1000_at 7.574620177
10010_at 6.382817285
100127886_at 4.583004535
100127972_at 5.357502504
100128008_at 6.569200074
100128062_at 10.27707872
100128414_at 4.621082756
100128919_at 7.02849767
100129015_at 5.075389049
100129128_at 5.28687916

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124278_D_133A_102_CB_1_C_p.cel.gz 3.2 Mb (ftp)(http) CEL
Processed data included within Sample table

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