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Sample GSM1124277 Query DataSets for GSM1124277
Status Public on Apr 20, 2013
Title D_133A_30_CB_1_C_p
Sample type RNA
 
Source name Post-mortem human brain tissue: Cerebellum
Organism Homo sapiens
Characteristics genechip assay site: D
original genechip type: U133P
subject id: 30
brain region: cerebellum
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.572345925
10001_at 6.852645672
10002_at 5.875881538
10003_at 4.756412207
100048912_at 6.933342667
10004_at 6.011169623
10005_at 7.134373666
10006_at 8.055287498
10007_at 7.277773817
10009_at 4.731250046
1000_at 7.712957187
10010_at 6.436591655
100127886_at 4.540784114
100127972_at 5.44683311
100128008_at 6.644099562
100128062_at 10.54288815
100128414_at 4.63223062
100128919_at 7.416685417
100129015_at 5.09011268
100129128_at 5.536740353

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124277_D_133A_30_CB_1_C_p.cel.gz 3.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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