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Sample GSM1124272 Query DataSets for GSM1124272
Status Public on Apr 20, 2013
Title D_133A_36_CB_0_C_p
Sample type RNA
 
Source name Post-mortem human brain tissue: Cerebellum
Organism Homo sapiens
Characteristics genechip assay site: D
original genechip type: U133P
subject id: 36
brain region: cerebellum
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.729334845
10001_at 7.121786079
10002_at 5.636156076
10003_at 4.91118497
100048912_at 7.076661144
10004_at 5.724815907
10005_at 7.392051227
10006_at 8.229838008
10007_at 7.263448105
10009_at 4.991602362
1000_at 7.809034783
10010_at 6.438703872
100127886_at 4.726686873
100127972_at 5.50453763
100128008_at 5.703345614
100128062_at 10.5000962
100128414_at 4.583809981
100128919_at 7.517334787
100129015_at 5.109037467
100129128_at 5.368262842

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124272_D_133A_36_CB_0_C_p.cel.gz 3.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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