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Sample GSM1124270 Query DataSets for GSM1124270
Status Public on Apr 20, 2013
Title D_133A_43_CB_1_C_p
Sample type RNA
 
Source name Post-mortem human brain tissue: Cerebellum
Organism Homo sapiens
Characteristics genechip assay site: D
original genechip type: U133P
subject id: 43
brain region: cerebellum
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 6.040156332
10001_at 7.242654513
10002_at 5.595107625
10003_at 4.868369693
100048912_at 6.63756375
10004_at 5.723250748
10005_at 7.263787555
10006_at 8.81776181
10007_at 7.284443466
10009_at 5.002062991
1000_at 7.715686648
10010_at 6.317463744
100127886_at 4.579150926
100127972_at 5.367695631
100128008_at 5.74212617
100128062_at 10.44548988
100128414_at 4.642776172
100128919_at 7.042716541
100129015_at 5.061107304
100129128_at 5.164310215

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124270_D_133A_43_CB_1_C_p.cel.gz 3.1 Mb (ftp)(http) CEL
Processed data included within Sample table

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