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Sample GSM1124264 Query DataSets for GSM1124264
Status Public on Apr 20, 2013
Title D_133A_110_CB_0_C
Sample type RNA
 
Source name Post-mortem human brain tissue: Cerebellum
Organism Homo sapiens
Characteristics genechip assay site: D
original genechip type: U133A
subject id: 110
brain region: cerebellum
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.725879186
10001_at 6.662643655
10002_at 5.98314834
10003_at 4.991016563
100048912_at 7.421123054
10004_at 5.818354212
10005_at 6.819855813
10006_at 8.141542085
10007_at 7.490324671
10009_at 4.399370355
1000_at 8.007762981
10010_at 6.14516796
100127886_at 4.780416536
100127972_at 5.572858483
100128008_at 6.62009102
100128062_at 10.73316578
100128414_at 4.622708637
100128919_at 7.147511188
100129015_at 5.415765276
100129128_at 5.406231026

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124264_D_133A_110_CB_0_C.cel.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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