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Sample GSM1124211 Query DataSets for GSM1124211
Status Public on Apr 20, 2013
Title M_133A_37_AnCg_1_C
Sample type RNA
 
Source name Post-mortem human brain tissue: Anterior Cingulate
Organism Homo sapiens
Characteristics genechip assay site: M
original genechip type: U133A
subject id: 37
brain region: anterior cingulate
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.759333813
10001_at 6.538861492
10002_at 5.465238634
10003_at 5.24310561
100048912_at 7.119864177
10004_at 5.643384081
10005_at 8.265028623
10006_at 8.444653802
10007_at 8.078273813
10009_at 4.848805283
1000_at 8.671979933
10010_at 5.859380137
100127886_at 4.794299506
100127972_at 5.929645035
100128008_at 7.793835601
100128062_at 9.287212606
100128414_at 4.778548882
100128919_at 7.019261426
100129015_at 5.324124967
100129128_at 5.296696319

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124211_M_133A_37_AnCg_1_C.cel.gz 3.5 Mb (ftp)(http) CEL
Processed data included within Sample table

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