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Sample GSM1124197 Query DataSets for GSM1124197
Status Public on Apr 20, 2013
Title I_133A_22_AnCg_1_C_p
Sample type RNA
 
Source name Post-mortem human brain tissue: Anterior Cingulate
Organism Homo sapiens
Characteristics genechip assay site: I
original genechip type: U133P
subject id: 22
brain region: anterior cingulate
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.362234652
10001_at 6.397922854
10002_at 5.555308884
10003_at 5.636127803
100048912_at 6.10591529
10004_at 5.647760787
10005_at 8.346363466
10006_at 8.998782717
10007_at 8.58296329
10009_at 4.725250499
1000_at 8.784989606
10010_at 5.958227141
100127886_at 4.759266646
100127972_at 5.263870925
100128008_at 7.770647564
100128062_at 10.12414094
100128414_at 4.801719118
100128919_at 7.26893028
100129015_at 5.341299427
100129128_at 5.179560679

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124197_I_133A_22_AnCg_1_C_p.cel.gz 3.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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