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Sample GSM1124163 Query DataSets for GSM1124163
Status Public on Apr 20, 2013
Title I_133A_21_AnCg_0_C_p
Sample type RNA
 
Source name Post-mortem human brain tissue: Anterior Cingulate
Organism Homo sapiens
Characteristics genechip assay site: I
original genechip type: U133P
subject id: 21
brain region: anterior cingulate
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.730601333
10001_at 6.463927758
10002_at 5.472860444
10003_at 5.48040093
100048912_at 6.379934919
10004_at 5.565560637
10005_at 8.037871891
10006_at 9.184488935
10007_at 8.68161704
10009_at 4.836691435
1000_at 8.738209282
10010_at 6.086353263
100127886_at 4.661499465
100127972_at 5.303791288
100128008_at 6.06037176
100128062_at 9.615437107
100128414_at 4.794000493
100128919_at 7.392486437
100129015_at 5.201074997
100129128_at 5.169427445

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124163_I_133A_21_AnCg_0_C_p.cel.gz 3.4 Mb (ftp)(http) CEL
Processed data included within Sample table

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