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Sample GSM1124155 Query DataSets for GSM1124155
Status Public on Apr 20, 2013
Title I_133A_106_AnCg_1_C
Sample type RNA
 
Source name Post-mortem human brain tissue: Anterior Cingulate
Organism Homo sapiens
Characteristics genechip assay site: I
original genechip type: U133A
subject id: 106
brain region: anterior cingulate
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.683615584
10001_at 6.361258185
10002_at 5.6475045
10003_at 5.147909019
100048912_at 7.035677179
10004_at 5.788225767
10005_at 8.117632066
10006_at 8.120127963
10007_at 8.253063316
10009_at 4.868359636
1000_at 8.901030266
10010_at 5.882179424
100127886_at 4.838514612
100127972_at 5.570999229
100128008_at 7.137303747
100128062_at 9.19437642
100128414_at 4.774048143
100128919_at 7.631378374
100129015_at 5.452870971
100129128_at 5.229720083

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124155_I_133A_106_AnCg_1_C.cel.gz 2.3 Mb (ftp)(http) CEL
Processed data included within Sample table

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