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Sample GSM1124150 Query DataSets for GSM1124150
Status Public on Apr 20, 2013
Title I_133A_37_AnCg_1_C
Sample type RNA
 
Source name Post-mortem human brain tissue: Anterior Cingulate
Organism Homo sapiens
Characteristics genechip assay site: I
original genechip type: U133A
subject id: 37
brain region: anterior cingulate
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.529731733
10001_at 6.681254087
10002_at 5.604839627
10003_at 5.399683034
100048912_at 6.621250514
10004_at 5.662549593
10005_at 8.729244158
10006_at 8.491523495
10007_at 8.358676913
10009_at 4.801224315
1000_at 8.773759714
10010_at 5.833306464
100127886_at 4.843321846
100127972_at 5.524997993
100128008_at 7.549106951
100128062_at 9.675844379
100128414_at 4.792393025
100128919_at 7.339957326
100129015_at 5.528132025
100129128_at 5.209822809

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124150_I_133A_37_AnCg_1_C.cel.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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