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Sample GSM1124136 Query DataSets for GSM1124136
Status Public on Apr 20, 2013
Title I_133A_52_AnCg_1_C
Sample type RNA
 
Source name Post-mortem human brain tissue: Anterior Cingulate
Organism Homo sapiens
Characteristics genechip assay site: I
original genechip type: U133A
subject id: 52
brain region: anterior cingulate
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.532146277
10001_at 6.424752607
10002_at 5.74155215
10003_at 5.230272644
100048912_at 7.037210451
10004_at 5.672431427
10005_at 8.357183013
10006_at 7.815226696
10007_at 8.144925355
10009_at 4.895842666
1000_at 8.75906711
10010_at 5.659283513
100127886_at 4.82993481
100127972_at 5.644513482
100128008_at 7.753487058
100128062_at 8.799839891
100128414_at 4.846478639
100128919_at 7.495769685
100129015_at 5.641372888
100129128_at 5.340097306

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124136_I_133A_52_AnCg_1_C.cel.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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