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Sample GSM1124122 Query DataSets for GSM1124122
Status Public on Apr 20, 2013
Title I_133A_30_Amy_1_C
Sample type RNA
 
Source name Post-mortem human brain tissue: Amygdala
Organism Homo sapiens
Characteristics genechip assay site: I
original genechip type: U133A
subject id: 30
brain region: amygdala
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.282415198
10001_at 5.854532588
10002_at 5.676573312
10003_at 5.025148289
100048912_at 6.587576216
10004_at 5.460092362
10005_at 7.764936065
10006_at 7.570241654
10007_at 8.054391789
10009_at 4.631340486
1000_at 8.25379377
10010_at 5.422923068
100127886_at 4.472886638
100127972_at 5.461241786
100128008_at 7.154128256
100128062_at 8.850936136
100128414_at 4.543329013
100128919_at 7.253064902
100129015_at 5.160118364
100129128_at 5.286539136

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124122_I_133A_30_Amy_1_C.cel.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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