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Sample GSM1124118 Query DataSets for GSM1124118
Status Public on Apr 20, 2013
Title I_133A_36_Amy_0_C
Sample type RNA
 
Source name Post-mortem human brain tissue: Amygdala
Organism Homo sapiens
Characteristics genechip assay site: I
original genechip type: U133A
subject id: 36
brain region: amygdala
Extracted molecule total RNA
Extraction protocol Trizol extraction of total RNA was performed according to the manufacturer's instructions.
Label biotin
Label protocol Biotinylated cRNA were prepared according to the standard Affymetrix protocol from 6 ug total RNA (Expression Analysis Technical Manual, 2001, Affymetrix).
 
Hybridization protocol Following fragmentation, 10 ug of cRNA were hybridized for 16 hr at 45C on GeneChip U133A Array. GeneChips were washed and stained in the Affymetrix Fluidics Station 400.
Scan protocol GeneChips were scanned using the Hewlett-Packard GeneArray Scanner G3000.
Data processing The data were analyzed with the RMA method using custom CDF file hgu133ahsentrezg.cdf.
 
Submission date Apr 17, 2013
Last update date Apr 20, 2013
Contact name Andy W Lin
E-mail(s) andylin@umich.edu
Organization name University of Michigan
Department MBNI
Lab Microarray
Street address 205 Zina Pitcher Place
City Ann Arbor
State/province MI
ZIP/Postal code 48103
Country USA
 
Platform ID GPL17027
Series (1)
GSE45642 Circadian patterns of gene expression in the human brain and disruption in major depressive disorder [control set]

Data table header descriptions
ID_REF
VALUE RMA

Data table
ID_REF VALUE
10000_at 5.174801872
10001_at 5.897988486
10002_at 5.580034821
10003_at 4.768241895
100048912_at 6.699506738
10004_at 5.374927778
10005_at 7.528941727
10006_at 8.623843678
10007_at 7.728389961
10009_at 4.525711087
1000_at 8.380308848
10010_at 5.42902537
100127886_at 4.482028689
100127972_at 5.428282414
100128008_at 6.969214411
100128062_at 9.01481302
100128414_at 4.603729976
100128919_at 7.222902577
100129015_at 5.187580848
100129128_at 5.072104826

Total number of rows: 11979

Table truncated, full table size 238 Kbytes.




Supplementary file Size Download File type/resource
GSM1124118_I_133A_36_Amy_0_C.cel.gz 3.6 Mb (ftp)(http) CEL
Processed data included within Sample table

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