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Sample GSM112327 Query DataSets for GSM112327
Status Public on Jun 06, 2006
Title SCC-012 cells transfected with control vector
Sample type RNA
Source name SCC-012 cells transfected with control vector
Organism Homo sapiens
Characteristics head and neck squamous cell carcinoma

Treatment protocol Cells were transfected using Fugene 6 (Roche, Indianapolis, IN). Cells were selected with hygromycin B 48 h after transfection and harvested for mRNA isolation.
Growth protocol Cells were grown in RPMI+10% FBS at 37C and harvested for mRNA after selection with Hygromycin
Extracted molecule polyA RNA
Extraction protocol mRNA isolation was performed as described in Flatt, P. M., Tang, L. J., Scatena, C. D., Szak, S. T., and Pietenpol, J. A. (2000) Mol Cell Biol 20, 4210-4223
Label biotin
Label protocol 300 ng of mRNA was reverse transcribed to double-stranded (ds) cDNA using an oligo-dT primer coupled to a T7 promoter. In vitro transcription from the ds cDNA was carried out using T7 polymerase and incorporating biotin-modified CTP and UTP ribonucleotides.
Hybridization protocol biotinylated cRNA (15µg) was fragmented and hybridized to the Affymetrix GeneChip U133 Plus 2.0. Hybridized cRNAs were washed and detected via streptavidin coupled to phycoerythrin using the Affymetrix 450 Fluidics Station and recommended protocols.
Scan protocol Results were visualized by laser scanner (Affymetrix GeneChip Scanner 3000).
Description Gene expression data on Affy HG133 Plus 2.0 from SCC-012 cells transfected with control vector
Data processing The data were analyzed with Microarray Suite version 5.0 (MAS 5.0) using Affymetrix default analysis settings and global scaling as normalization method. The trimmed mean target intensity of each array was arbitrarily set to 100.
Submission date Jun 05, 2006
Last update date Jun 05, 2006
Contact name Jennifer Pietenpol
Organization name Vanderbilt University Medical Center
Department Biochemistry
Lab 652 PRB
Street address 23rd Ave South at Pierce
City Nashville
State/province TN
ZIP/Postal code 37232
Country USA
Platform ID GPL570
Series (1)
GSE4975 Expression data from p63 siRNA in squamous cell lines

Data table header descriptions
VALUE MAS5-calculated Signal intensity
ABS_CALL the call in an absolute analysis that indicates if the transcript was present (P), absent (A), marginal (M), or no call (NC)

Data table
AFFX-BioB-5_at 397.5 P
AFFX-BioB-M_at 494 P
AFFX-BioB-3_at 191.9 P
AFFX-BioC-5_at 814.5 P
AFFX-BioC-3_at 605.3 P
AFFX-BioDn-5_at 1209.1 P
AFFX-BioDn-3_at 3882.6 P
AFFX-CreX-5_at 9642.2 P
AFFX-CreX-3_at 12535.9 P
AFFX-DapX-5_at 43.9 A
AFFX-DapX-M_at 58.4 A
AFFX-DapX-3_at 12.8 A
AFFX-LysX-5_at 67.2 A
AFFX-LysX-M_at 54.1 A
AFFX-LysX-3_at 28.7 A
AFFX-PheX-5_at 12 A
AFFX-PheX-M_at 14.8 A
AFFX-PheX-3_at 34.1 A
AFFX-ThrX-5_at 6.8 A
AFFX-ThrX-M_at 13.8 A

Total number of rows: 54675

Table truncated, full table size 981 Kbytes.

Supplementary data files not provided

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