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Sample GSM1122285 Query DataSets for GSM1122285
Status Public on May 02, 2014
Title iPS-derived neurons_HSV-1 quiescient infection 2
Sample type RNA
Source name iPS-derived neurons_HSV-1 quiescient infection
Organism Homo sapiens
Characteristics tissue source: human skin biopsy samples
cells: glutamatergic neurons
cell type: human iPS-derived neurons
gender: male
age (yrs): 26.6
Treatment protocol Neuronal cultures were either infected with HSV-1 in triplicate or uninfected, with each individual sample harvested separately to provide biological replicates for expression analysis. Microarray analysis was performed on i) uninfected neurons; ii) neurons infected with HSV-1 at an MOI of 0.3 in the presence or absence of (E)-5-(2-bromovinyl)-2'-deoxyuridine along with interferon (IFN)-α; iii) neuron quiescent cultures treated with sodium butyrate (NaB, 5 mM); iv) uninfected neurons treated with E)-5-(2-bromovinyl)-2'-deoxyuridine and interferon IFN-α; v) uninfected neurons treated with NaB
Extracted molecule total RNA
Extraction protocol RNA was extracted using RNeasy kits (Qiagen)
Label Cy3-CTP
Label protocol Cy3-CTP labeled RNA was prepared according to the standard Agilent protocol from 50ng total RNA.
Hybridization protocol Labeled RNA was hybridized for 17 hr at 65C on Agilent gene expression array. Arrays were washed according to the manufacturer's protocol.
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505B) using one color scan setting for 8X60K array slides
Description iPS-derived neurons
Data processing Agilent Feature Extraction v10.7.3.1 default parameters and further analyzed by a cyclic loess algorithm authored in the R programming environment using the Bioconductor suite of tools, and only probes with Entrez gene IDs are selected for further analysis (in total 38804).
Submission date Apr 15, 2013
Last update date May 02, 2014
Contact name Leonardo D'Aiuto
Phone 4126570558
Organization name UPMC
Street address 3811 O’Hara Street
City Pittsburgh
State/province PA
ZIP/Postal code 15213
Country USA
Platform ID GPL17077
Series (2)
GSE46041 Human cellular models for Herpes Simplex Virus, type 1 (HSV-1) using induced pluripotent stem cell derived neuronal lineages
GSE46043 Induced pluripotent stem cell derived neuronal lineages as human cellular models for Herpes Simplex Virus, type 1 (HSV-1) infections

Data table header descriptions
VALUE Normalized signal intensity

Data table
A_19_P00315518 2.517276796
A_19_P00315519 3.528333426
A_19_P00315528 4.264399418
A_19_P00315529 3.620774434
A_19_P00315601 10.8247952
A_19_P00315603 10.29489076
A_19_P00315625 3.517110739
A_19_P00315627 6.974063776
A_19_P00315631 2.69040375
A_19_P00315691 5.534682013
A_19_P00315810 6.786148373
A_19_P00315826 6.386447699
A_19_P00315841 5.095785386
A_19_P00315863 3.946919182
A_19_P00315905 2.409828394
A_19_P00315914 6.505186539
A_19_P00315936 7.861489156
A_19_P00315967 6.850385827
A_19_P00316035 2.777420316
A_19_P00316159 15.9378096

Total number of rows: 38804

Table truncated, full table size 957 Kbytes.

Supplementary file Size Download File type/resource
GSM1122285_HSV-1_quiescient_infection_2.txt.gz 3.0 Mb (ftp)(http) TXT
Processed data included within Sample table

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