|
| Status |
Public on Apr 12, 2013 |
| Title |
Bmi1KO.2088 |
| Sample type |
RNA |
| |
|
| Source name |
Mouse incisor dental epithelia
|
| Organism |
Mus musculus |
| Characteristics |
genotype: Bmi1-/-
tissue: incisor dental epithelia
genetic background: mixed
|
| Extracted molecule |
total RNA |
| Extraction protocol |
Whole incisors were incubated in accumax, dental epithlial were mechanically separated from mesenchyme and total RNA was extracted using the Qiagen Rneasy mini kit.
|
| Label |
Cy3
|
| Label protocol |
standard Agilent protocol
|
| |
|
| Hybridization protocol |
Hybridizations were performed for 17 hrs, according to the manufacturers protocol.
|
| Scan protocol |
Arrays were scanned using the Agilent microarray scanner and raw signal intensities were extracted with Feature Extraction v10.6 software.
|
| Data processing |
This dataset was normalized using the quantile normalization method that is proposed by Bolstad et al (2003). No background subtraction was performed, and the median feature pixel intensity was used as the raw signal before normalization.
|
| |
|
| Submission date |
Apr 11, 2013 |
| Last update date |
Apr 18, 2013 |
| Contact name |
Brian Biehs |
| E-mail(s) |
brianbiehs@gmail.com
|
| Organization name |
UCSF
|
| Street address |
505 Parnassus Ave
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
|
| Platform ID |
GPL11641 |
| Series (2) |
|
