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Sample GSM1118248 Query DataSets for GSM1118248
Status Public on Sep 16, 2013
Title NOA_POST_4
Sample type RNA
 
Source name testicular biopsy with nonobstructive azoospermia
Organism Homo sapiens
Characteristics Sex: male
condition: NOA
tissue: testis
testis region: right
age (yrs): 31
histopatological description: postmeiotic arrest
Treatment protocol Not applicable, samples extracted from untreated patients with various stages of azoospermia
Growth protocol Not applicable, total RNA extracted from testicular biopsy cells
Extracted molecule total RNA
Extraction protocol Tri-Reagent (Sigma-Aldrich, St. Louis, USA) and post-purification using RNeasy Plus Mini Kit (Qiagen, Hilden, Germany)
Label biotin
Label protocol The sense-strand cDNA was fragmentized and labeled using the Affymetrix GeneChip® WT Terminal Labeling Kit (Affymetrix, Santa Clara, CA).
 
Hybridization protocol Array hybridization, washing, and scanning were performed according to manufacturer’s recommendations by using GeneChip® Hybridization, Wash and Stain Kit (Affymetrix, Santa Clara, CA).
Scan protocol Microarrays scanned using Affymetrix 3000 Scanner according to the instructions of the manufacturer
Description Biomarkers project
Data processing Raw data were normalized with RMA method using Affymetrix software delivered to the scanner.
 
Submission date Apr 09, 2013
Last update date Sep 24, 2013
Contact name Tomasz Stokowy
E-mail(s) tomasz.stokowy@uib.no
Organization name University of Bergen
Department IT Division
Lab Scientific Computing
Street address Nygardsgaten 5
City Bergen
ZIP/Postal code 5020
Country Norway
 
Platform ID GPL6244
Series (1)
GSE45885 Potential biomarkers of non-obstructive azoospermia identified in microarray gene expression analysis
Relations
Reanalyzed by GSM1238972
Reanalyzed by GSM1238978

Data table header descriptions
ID_REF
VALUE rma-gene-default-Signal

Data table
ID_REF VALUE
7896736 5.626174
7896738 2.595501
7896740 2.689371
7896742 9.14663
7896744 7.417861
7896746 8.796126
7896748 5.463706
7896750 2.83942
7896752 8.894821
7896754 6.863338
7896756 4.033456
7896759 5.496349
7896761 5.06356
7896779 5.342267
7896798 4.97637
7896817 4.683592
7896822 6.332732
7896859 3.397401
7896861 2.86339
7896863 4.494775

Total number of rows: 33297

Table truncated, full table size 549 Kbytes.




Supplementary file Size Download File type/resource
GSM1118248_8P_HuGene-1_0-st-v1_.CEL.gz 3.7 Mb (ftp)(http) CEL
Processed data included within Sample table

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