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Sample GSM1116447 Query DataSets for GSM1116447
Status Public on Apr 01, 2016
Title Cd117-Cd27+Cd11b-NK_rep2
Sample type RNA
 
Source name NK_Cd117-Cd27+Cd11b-
Organism Mus musculus
Characteristics strain: C57Bl/6
gender: female
tissue: spleen
cell type: NK cells
cell subtype: Cd117-Cd27+Cd11b-
Growth protocol Prior to MoFlo sorting, single cell suspensions were stained with the following antibodies: FITC-conjugated anti-CD11b (M1/70) (Miltenyi, Bergisch Gladbach, Germany), PerCP-conjugated anti-CD3ε (145-2C11) and PerCP-conjugated anti-CD19 (1D3) were purchased from BioLegend (San Diego, CA, USA), APC-conjugated anti-CD117 (c-kit) (2B8), PE-conjugated anti-CD27 (LG.3A11), PE-Cy7-conjugated anti-NK1.1 (PK136) and BD Horizon V450 conjugated anti-CD45.1 (A20) were purchased from BD Biosciences (Heidelberg, Germany). NK cell purity, functional properties and subset distribution was analyzed by FACS Canto II (BD Biosciences, Heidelberg, Germany) and use of the FlowJo Software (Treestar, Ashland, Oregon, USA).
Extracted molecule total RNA
Extraction protocol RNA was isolated from freshly sorted NK subsets using the RNAeasy Micro Kit (QIAGEN, Hilden, Germany) according to the manufacturer’s instructions. RNA quality was checked using the Agilent 2100 Bioanalyzer.
Label Cy3
Label protocol High-quality RNA were amplified and Cyanine 3-CTP-labelled with the one colour low RNA input linear amplification kit (Agilent).
 
Hybridization protocol Labeling efficiency was controlled using the NanoDrop spectrophotometer and labeled cRNA were fragmented and hybridized on whole mouse genome expression arrays (4 × 44 K Agilent). Microarrays were washed and subsequently scanned with an Agilent scanner.
Scan protocol Microarrays were washed and subsequently scanned with an Agilent scanner.
Description SAMPLE 4
Data processing Raw data were extracted with Feature Extraction 9.5.1 software and analyzed using GeneSpring GX 11 (Agilent).
 
Submission date Apr 08, 2013
Last update date Apr 01, 2016
Contact name Michael Rehli
E-mail(s) michael.rehli@klinik.uni-r.de
Organization name University Hospital Regensburg
Department Internal Med III
Street address F.-J.-Strauss-Allee 11
City Regensburg
ZIP/Postal code 93042
Country Germany
 
Platform ID GPL7202
Series (1)
GSE45842 Expression profiles of mouse NK cell subsets

Data table header descriptions
ID_REF
VALUE Normalized signal intensity (log2 scale)

Data table
ID_REF VALUE
(-)3xSLv1 -8.62056
(+)E1A_r60_1 8.896109
(+)E1A_r60_3 -6.2244577
(+)E1A_r60_a104 -6.5289598
(+)E1A_r60_a107 -3.084261
(+)E1A_r60_a135 -0.45091057
(+)E1A_r60_a20 0.555933
(+)E1A_r60_a22 2.1808634
(+)E1A_r60_a97 4.4489813
(+)E1A_r60_n11 6.342718
(+)E1A_r60_n9 7.2738304
(+)eQC-39 -3.9024572
(+)eQC-40 -8.415556
(+)eQC-41 -7.9204373
(+)eQC-42 -4.723131
A_51_P100021 -4.868724
A_51_P100034 3.2179766
A_51_P100052 -8.520458
A_51_P100063 -4.949959
A_51_P100084 -3.4606118

Total number of rows: 41282

Table truncated, full table size 939 Kbytes.




Supplementary file Size Download File type/resource
GSM1116447_US94700331_251486826578_S01_GE1_107_Sep09_1_2_18_Cd27.txt.gz 2.2 Mb (ftp)(http) TXT
Processed data included within Sample table

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