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Sample GSM1113426 Query DataSets for GSM1113426
Status Public on Apr 04, 2013
Title Serum_control_pooled, rep1
Sample type RNA
Source name Venous blood, control
Organism Homo sapiens
Characteristics tissue: blood serum
disease status: control
Treatment protocol The whole blood was separated into serum and cellular fractions by centrifugation at 4,000 rpm for 10 min, followed by 12000 rpm for 15 min to completely remove cell debris. To detect the generalizable signatures of serum miRNAs for MMD, we pooled serum samples from 10 patients and 10 controls.
Extracted molecule total RNA
Extraction protocol Trizol Reagent (Invitrogen, Carlsbad, CA) was used for serum denaturizing and the Qiagen miRNeasy Mini kit (Qiagen, Valencia, CA) for RNA collection and purification according to the manufacturer's protocol.
Label Cy3
Label protocol Cyanine-3 (Cy3) labeled cRNA was prepared from 100 ng RNA using Agilent's miRNA Complete Labeling and Hyb Kit (p/n 5190-0456) according to the manufacturer's instructions.
Hybridization protocol Agilent Human 8 x 60K miRNA Array was performed on the two pooled samples. Hybridization was carried out according to the instructions of the Agilent's miRNA Complete Labeling and Hyb Kit (p/n 5190-0456).
Scan protocol Slides were scanned immediately after washing on the Agilent DNA Microarray Scanner (G2505C) using one color scan setting for 8x60k array slides (Scan Area 61x21.6 mm, Scan resolution 5um, Dye channel is set to Green and Green PMT is set to 100%).
Description Serum samples from 10 controls were pooled into a single control sample.
Data processing Data were acquired using the Agilent G2565BA Microarray Scanner System and Agilent Feature Extraction Software (Scan resolution 5μm, PMT 100%). Probe intensities were normalized using GeneSpring 12.0 through percentile shift normalization. Differentially expressed miRNAs were identified through Fold Change filtering (Fold change >2).
Submission date Apr 03, 2013
Last update date Apr 04, 2013
Contact name Dongwei Dai
Organization name Changhai Hospital
Street address 168 Changhai Road
City Shanghai
ZIP/Postal code 200433
Country China
Platform ID GPL15159
Series (1)
GSE45737 Determination of the serum miRNA expression profile in human moyamoya disease (MMD)

Data table header descriptions
VALUE Normalized signal intensity

Data table
A_25_P00010019 1.448
A_25_P00010020 0.818
A_25_P00010021 1.201
A_25_P00010023 7.237
A_25_P00010041 0.593
A_25_P00010042 null
A_25_P00010043 null
A_25_P00010044 null
A_25_P00010047 1.462
A_25_P00010048 null
A_25_P00010053 0.465
A_25_P00010054 1.111
A_25_P00010062 null
A_25_P00010063 0.057
A_25_P00010070 15.835
A_25_P00010071 10.924
A_25_P00010072 4.771
A_25_P00010073 null
A_25_P00010078 5.556
A_25_P00010079 0.018

Total number of rows: 3523

Table truncated, full table size 70 Kbytes.

Supplementary file Size Download File type/resource
GSM1113426_control.txt.gz 5.1 Mb (ftp)(http) TXT
Processed data included within Sample table

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