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Status |
Public on Nov 04, 2013 |
Title |
genomic DNA from differentiated, untransduced H9 hESC, replicate B |
Sample type |
genomic |
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|
Source name |
differentiated_untransduced H9 hESC
|
Organism |
Homo sapiens |
Characteristics |
cell line: H9 cell type: Cultured hESC genotype/variation: untransduced control
|
Extracted molecule |
genomic DNA |
Extraction protocol |
genomic DNA was extracted and purified from cultured cells using Gentra Puregene DNA extraction Kit according to standard instructions
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Label |
Cy5 and Cy3
|
Label protocol |
Standard Illumina Protocol
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|
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Hybridization protocol |
bisulphite converted DNA was amplified, fragmented and hybridised to Illumina Human Methylation450 Beadchip using standard Illumina protocol
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Scan protocol |
Arrays were imaged using BeadArray Reader using standard recommended Illumina scanner setting
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Description |
samples were hybridized on to 2 different arrays, this sample was done on array 2 H9-4B
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Data processing |
non_normalized_array*.txt contains unmethylated and methylated signal intensities Illumina Genome Studio Methylation data were imported into the Illumina Genome Studio software and converted to a Partek Project File (.ppj) with the Partek Methylation Module GenomeStudio Data Analysis Software’s Gene Expression Module (GSGX) Version 1.1.0
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Submission date |
Apr 03, 2013 |
Last update date |
Nov 04, 2013 |
Contact name |
Sangeetha Kaveri Mahadevan |
Organization name |
Baylor College of Medicine
|
Department |
Obsterics and Gynecology
|
Lab |
Ignatia Van den Veyver
|
Street address |
1250 Moursund St, Room 1025
|
City |
Houston |
State/province |
Texas |
ZIP/Postal code |
77030 |
Country |
USA |
|
|
Platform ID |
GPL13534 |
Series (1) |
GSE45727 |
NLRP7 promotes trophoblast lineage differentiation, YY1 binding and alters CpG methylation |
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